Tick identification alabama3/7/2024 ![]() Ticks are distributed widely across the world, and approximately 10% of the currently known 867 tick species act as arthropod vectors in the transmission of human and animal pathogens ( Zhang et al., 2019 Yang et al., 2021). ![]() microplus in Yunnan into MLVA genotype 1, which is closely related to previously described genotypes found primarily in tick and human samples from different regions of the globe, indicating a potential public health threat posed by C. Multilocus variable number tandem repeat analysis with 6 loci (MLVA-6) discriminated the C. ![]() microplus in Yunnan province of China, reporting for the first time that C. The results provide insight into the diversity of TBPs and their coinfections in Rh. marginale, and CLE were most frequent and detected in 3.68% (19/516) and 3.10% (16/516) of these ticks, respectively. marginale and the triple coinfection with Ca. The pooled DNA samples of these ticks were evaluated using metagenomic next-generation sequencing (mNGS) and then TBPs in individual ticks were identified using genus- or group-specific nested polymerase chain reaction (PCR) combined with DNA sequencing assay. microplus ( n = 516) feeding on cattle were collected. microplus in Yunnan and to analyze the phylogenetic characterization of TBPs detected in these ticks. The aim of this study was to detect TBPs in Rh. However, few reports on the prevalence of tick-borne pathogens (TBPs) in Rh. Rhipicephalus microplus, a vector that can transmit many pathogens to humans and domestic animals, is widely distributed in Yunnan province, China.
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